H2N2V1, 1957, Analysis, bibRecord, 000145

Adaptation of Influenza A Viruses to Cells Expressing Low Levels of Sialic Acid Leads to Loss of Neuraminidase Activity

Identifieur interne : 000145 ( 1957/Analysis ); précédent : 000144; suivant : 000146

Adaptation of Influenza A Viruses to Cells Expressing Low Levels of Sialic Acid Leads to Loss of Neuraminidase Activity

Auteurs : Mark T. Hughes ; Martha Mcgregor ; Takashi Suzuki ; Yasuo Suzuki ; Yoshihiro Kawaoka [États-Unis]

Source :

Journal of Virology [ 0022-538X ] ; 2001.

RBID : PMC:114867

Abstract

Influenza A viruses possess two virion surface proteins, hemagglutinin (HA) and neuraminidase (NA). The HA binds to sialyloligosaccharide viral receptors, while the NA removes sialic acids from the host cell and viral sialyloligosaccarides. Alterations of the HA occur during adaptation of influenza viruses to new host species, as in the 1957 and 1968 influenza pandemics. To gain a better understanding of the contributions of the HA and possibly the NA to this process, we generated cell lines expressing reduced levels of the influenza virus receptor determinant, sialic acid, by selecting Madin-Darby canine kidney cells resistant to a lectin specific for sialic acid linked to galactose by α(2-3) or α(2-6) linkages. One of these cell lines had less than 1/10 as much N-acetylneuraminic acid as its parent cell line. When serially passaged in this cell line, human H3N2 viruses lost sialidase activity due to a large internal deletion in the NA gene, without alteration of the HA gene. These findings indicate that NA mutations can contribute to the adaptation of influenza A virus to new host environments and hence may play a role in the transmission of virus across species.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC114867

DOI: 10.1128/JVI.75.8.3766-3770.2001
PubMed: 11264365
PubMed Central: 114867

Affiliations:

Links to Exploration step

PMC:114867

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Adaptation of Influenza A Viruses to Cells Expressing Low Levels of Sialic Acid Leads to Loss of Neuraminidase Activity

Adaptation of Influenza A Viruses to Cells Expressing Low Levels of Sialic Acid Leads to Loss of Neuraminidase Activity

Source :

Abstract

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